Reporter Constructs and Cell Lines

Overview:

Specific, rapid, and convenient analysis of the in in vivo activation of transcriptional pathways.

Eukaryotic gene expression is regulated by a wide variety of developmental and environmental stimuli. First, an extracellular signaling molecule binds to a specific receptor. The signal is then transmitted through a series of molecular cascades, which activate or deactivate specific transcription factors (TFs) that regulate gene expression. The expression of any given gene is controlled by multiple transcription factors, which in turn are modulated by multiple signal transduction pathways. Many of these signal transduction pathways converge at transcription factors that bind to specific transcriptional response elements (TREs) found in the promoters of various genes and modulate the transcription of these genes. The activation of a signal transduction pathway (e.g. by growth factors, drugs, etc.) can therefore be monitored by the expression level of the reporter gene controlled by a promoter containing these response elements. The commonly used plasmid-based transcriptional reporter vectors containing different reporter genes can be delivered by transient transfection to the nucleus of target cells to monitor the activation of signal transduction pathways converging at a specific response element.

Lentiviral expression vectors are the most effective vehicles for delivering genetic material to almost any mammalian cell—including non-dividing cells and to model organisms. As with standard plasmid vectors, it is possible to introduce lentiviral Transcriptional Reporter (TR) constructs in plasmid form into the cells with low-to-medium efficiency using conventional transfection protocols. However, by packaging the lentiviral TR vector construct in pseudoviral particles, you can obtain highly efficient transduction and heritable expression of transcriptional reporter constructs—even with the most difficult-to-transfect cells, like primary, stem, and differentiated cells. In comparison to retroviral delivery systems, lentivectors enter the cell nucleus without requiring cell replication.

Create your own reporter cell line with our ready-made lentivirus or choose from our clonal reporter cell lines.

Clonal cell lines isolated and characterized for accurate transcriptional reporting using lentiviral expression vectors. As with standard plasmid vectors, it is possible to introduce lentiviral Transcriptional Reporter (TR) constructs in plasmid form into the cells with low-to-medium efficiency using conventional transfection protocols. However, by packaging the lentiviral TR vector construct in pseudoviral particles, you can obtain highly efficient transduction and heritable expression of transcriptional reporter constructs—even with the most difficult-to-transfect cells, like primary, stem, and differentiated cells. In comparison to retroviral delivery systems, lentivectors enter the cell nucleus without requiring cell replication.