The MicroRNA Target Selection System

Overview:

he miR-Selection lentivector features a dual reporter system with firefly luciferase (Fire) and a cytotoxic sensor (Ctx). The miR-Selection platform captures the 3’ UTR to microRNA binding event using a survival screen by modulating the reduction of the cytotoxic sensor. Quantitative validation is made simple using the built-in luciferase reporter. This powerful and elegant technology finally enables the accurate identification of microRNA targets and enables high-throughput target identification screens.

Clone your 3'UTR into the vector - easily identify the microRNAs that bind
target selection

miR-Selection Lentivector details
• Total size of the lentivector = 10.2Kb
• Sequence of the Multiple Cloning Site (MCS)
5'-GAATTCGCTAGCTGAGGCAGCAGAGACCGGCCGGCCGGTCTCTCTCT-GTTCGAGGATCCCTCGAGGTCGACGCGGCCGC-3'