Transcription Reporter Cloning System Details

The easiest method for efficient & accurate cloning of Transcription Response Elements or Promoter sequences with ready-to-use (pre-linearized) Clone-it vectors that require no restriction digestion.

Features and Benefits of the Clone-it™ Cloning System
* Ligase, recombinase, topoisomerase and restriction enzyme-free system
* Rapid and Directional cloning with no recombination errors
* Near 100% cloning efficiency
* Short hands-on time and adaptable for high-throughput cloning
* Lentiviral-based expression systems containing HIV backbone for high viral titers
* Reliable delivery to dividing or non-dividing cells
* Convenient sorting of transfected or transduced cells
* Easily create stable cell lines

Transcription Reporter Cloning System Details

Transcription Response Element or Promoter sequences are cloned downstream of an inactive, minimal CMV ("mCMV") promoter. Transcriptional activity driven by your promoter sequences generate a robust fluorescent marker response. Positively expressing cells can be sorted using a RFP reporter (Cat# LF528A-1).

Clone-it Reporter Vector Map

Sample Transcription Reporter Data

Murine neural stem cells reporting BDNF promoter transactivation four weeks after differentiation. Visualization of single cells (left) and neurosphere (right).

Details of the Lentiviral Delivery System

* Expression in target cells through transfection (plasmid form) or transduction (packaged constructs).
* Simple packaging into pseudoviral particles with SBI’s pPACK Lentivector Packaging System.)
* Deliver expression constructs into a wide range of cell lines including non-dividing or difficult-to-transfect cell lines with biologically-safe and high-titer enabled HIV-based lentivectors.

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