MicroRNA Precursor Cloning System Details

Description
MicroRNA precursor genes are cloned into the 3' end of the copGFP or RFP transcript where they can be processed naturally into precursor and mature microRNAs. Positively expressing cells can be sorted using a GFP or RFP reporter.

Cloning Efficiency Data

ample MicroRNA Expression Data

ransfected into 293 cells with the indicated amount of plasmid DNA. Mature miR-10b was measured using QuantiMir™.

Details of the Lentiviral Delivery System

* Expression in target cells through transfection (plasmid form) or transduction (packaged constructs).
* Simple packaging into pseudoviral particles with SBI’s pPACK Lentivector Packaging System.
* Deliver expression constructs into a wide range of cell lines including non-dividing or difficult-to-transfect cell lines with biologically-safe and high-titer enabled HIV-based lentivectors.

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