cDNA Cloning System Details

Seamlessly clone your cDNA without the worries of internal restriction enzyme sites using SBI's Clone-it cDNA vectors. This novel system enables the rapid cloning of any cDNA up to 5kb. Highly active EF1α promoter drives transcription, and Kozak elements are built-in for optimized translation. Proteins can optionally be expressed with N-terminal HA epitope or C-terminal T2A tag. RFP reporter (Cat# LF520A-1) or Puromycin resistance gene (Cat# LF521A-1) are coexpressed for sorting or selection.

Clone-it cDNA Vector Map

Cloning Efficiency Data

Accurate & Directional Cloning

Monitor positive expressing cells using RFP reporter

Easily create stable cell lines expressing your cDNA.

The cDNA constructs can be used in transient transfection experiments (shown left) or can be used to package lentiviral particles. The particles can then be used to transduce any cell type to create stable cell lines permanently expressing the protein of interest. Examples of VSV-g pseudotyped lentiviral transductions are also shown.
RFP Cell Sorting or Puromycin Selection.

Details of the Lentiviral Delivery System

* Expression in target cells through transfection (plasmid form) or transduction (packaged constructs).
* Simple packaging into pseudoviral particles with SBI's pPACK Lentivector Packaging System
* Deliver expression constructs into a wide range of cell lines including non-dividing or difficult-to-transfect cell lines with biologically-safe and high-titer enabled HIV-based lentivectors.

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